Allopartis has expertise in the formation of complex proteins in droplets from single DNA molecules. A complete toolkit for manipulating DNA, substrate, and selection conditions provides the flexibility to optimize interactions across a range of proteins and substrates. These capabilities enable protein engineering at a scale and speed akin to that of single-molecule DNA sequencing. The transformation in medical, diagnostic, and molecular biology discovery made possible by next-generation DNA sequencing is now achievable in protein discovery and engineering. Example tunable protein characteristics include: increased activity, altered temperature optimum, temperature/time/pH stability, pH optimum, inhibitor tolerance, end product tolerance, expression level, and substrate/ligand affinity. The toolkit is also adept at soluble substrate targets and to new gene discovery from minute cellular or environmental samples without the need for cell culture. The AlloScreen is complemented by a downstream protein production and testing pipeline that further refines optimized proteins for pharmaceutical and industrial scale production.